What is Toluidine Blue (TB) Dye?
TB dye is a basic thiazine metachromatic dye, which has a high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. The epithelium of the external genitalia does not have nucleated cells and prevents contact of stain with nuclei. Where the epithelium is damaged and the underlying nucleated cells are exposed, the nuclei stain blue.
Does TB Dye interfere with evidence collection?
Toluidine Blue stain is easy and safe to use, available, inexpensive and does not interfere with medico-legal evidence. There are no adverse effects on DNA detection and identification wi